In North America, the muscadine is a native grape species with a thick skin and multiple seeds. The muscadine was the first native grape species to be cultivated in North America. In the wild, male varieties of the grape are the most prolific. If you've ever stumbled on a wild-growing muscadine, you have probably found a male variety of the plant. In your own garden or vineyard, the best way to propagate muscadine is through softwood cuttings.
Select vines for cutting from the parent plant that are healthy and vigorous with no outward signs of disease.
Cut the end of a vine away from the parent plant between the fifth and sixth leaves.
Divide the vine into 6-inch pieces with three or four places where leaves, tendrils or muscadine fruit stems emerge from the vine. This spot is known as a node. Make your cuts next to a node.
Place your cuttings in a bucket of lukewarm water and leave them there until they are ready to be transplanted.
Fill a planting tray with peat moss.
Remove all but the top two of the leaves from each cutting and insert the end of the cuttings half-way down into the peat moss.
Keep the peat moss moist until the vines take root.
Transplant the rooted cuttings to their permanent location once they begin to grow. Muscadine vines and their grapes dislike being moved, so be certain that the location you transplant them into will be their permanent location.